Coding

Part:BBa_K5048048:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


LAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 859
    Illegal EcoRI site found at 2206
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 859
    Illegal EcoRI site found at 2206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 859
    Illegal EcoRI site found at 2206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 859
    Illegal EcoRI site found at 2206
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 859
    Illegal EcoRI site found at 2206
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adhesion protein from non-pathogenic Listeria.


Source

Listeria innocua strain CFSAN044836 chromosome, complete genome,Sequence ID: CP045743.1,Range 1: 55424 to 58024


References

Drolia, R., Amalaradjou, M. A. R., Ryan, V., Tenguria, S., Liu, D., Bai, X., Bhunia, A. K. (2020). Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nature Communications, 11(1), 6344. doi:10.1038/s41467-020-20200-5